R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
NUP210 (E9A5G) Rabbit mAb #50047
Filter:
- WB
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 210 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
NUP210 (E9A5G) Rabbit mAb recognizes endogenous levels of total NUP210 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro290 of human NUP210 protein.
Background
The nuclear pore complex (NPC) is a multi-subunit protein channel that spans the nuclear envelope and is responsible for the nucleocytoplasmic trafficking of RNA, proteins, and ribonucleoproteins (1,2). Nucleoporin 210 kDa (NUP210), also referred to as nuclear pore glycoprotein 210 (gp210), is one of the three integral membrane proteins of the NPC that have been shown to link the complex to the nuclear envelope (3,4). NUP210 is recruited in later stages of the nuclear pore assembly and is expressed in a cell-type specific manner (5). NUP210 expression has been shown to be induced during myogenic and neural differentiation, and its addition to NPCs is needed for activation of essential cell differentiation genes (6). It has also been shown that NUP210 is required for proper muscle structural gene expression at NPCs by acting as a scaffold for the Mef2C transcriptional complex (7). More recently, NUP210 was identified as a metastasis susceptibility gene for estrogen receptor-positive breast cancer (8).
- Raices, M. and D'Angelo, M.A. (2017) Curr Opin Cell Biol 46, 26-32.
- Xu, S. and Powers, M.A. (2009) Semin Cell Dev Biol 20, 620-30.
- Stavru, F. et al. (2006) J Cell Biol 173, 509-19.
- Stavru, F. et al. (2006) J Cell Biol 173, 477-83.
- Olsson, M. et al. (2004) Exp Cell Res 292, 359-70.
- D'Angelo, M.A. et al. (2012) Dev Cell 22, 446-58.
- Raices, M. et al. (2017) Dev Cell 41, 540-554.e7.
- Amin, R. et al. (2021) Nat Commun 12, 7216.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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