R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
NUP153 (E3I6Z) Rabbit mAb #98559
Filter:
- WB
- IF
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 180 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:400 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
NUP153 (E3I6Z) Rabbit mAb recognizes endogenous levels of total NUP153 protein.
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro428 of human NUP153 protein.
Background
The nuclear pore complex (NPC) is a multi-subunit protein channel that spans the nuclear envelope and is responsible for the nucleocytoplasmic trafficking of RNA, proteins, and ribonucleoproteins (1,2). Nucleoporin 153 kDa (NUP153) protein functions as a scaffolding element that recruits other proteins to form the “nuclear basket” on the nuclear side of the pore complex. NUP153 is essential for normal nucleocytoplasmic transport of proteins and mRNAs and is critical for the quality control and retention of unspliced mRNAs in the nucleus (1,2). NUP153 is a potential DNA-binding subunit of the NPC and is important for proper regulation of embryonic stem cell pluripotency and differentiation (1,3). Depletion of NUP153 leads to derepression of developmental genes and induction of early differentiation. NUP153 binds to Nucleoporin-Associated Regions (NARs) that are found near the transcriptional start sites of developmental genes and mediates recruitment of polycomb repressor complex 1 (PRC1) to repress transcription in embryonic stem cells (3).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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