R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
NSUN5 (E5N7R) Rabbit mAb #34405
Filter:
- WB
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 52 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:200 - 1:400 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
NSUN5 (E5N7R) Rabbit mAb recognizes endogenous levels of total NSUN5 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His392 of human NSUN5 protein.
Background
NSUN5 was originally discovered as a yeast 5-methylcytosine methyltransferase that methylates C2278 of the 25S ribosomal RNA (rRNA) (1). It also methylates C3782 in human 28S rRNA, and contributes to protein synthesis and cell growth (2). NSUN5 is overexpressed in many cancer types, where it contributes to increased cellular proliferation (3,4). NSUN5 also plays an important role in cerebral cortex development, and NSUN5 mutants have neurological dysfunction (5-7).
- Schosserer, M. et al. (2015) Nat Commun 6, 6158.
- Heissenberger, C. et al. (2019) Nucleic Acids Res 47, 11807-11825.
- Zhou, J. et al. (2023) Mol Oncol 17, 1763-1783.
- Zhang, X.W. et al. (2022) Oncol Lett 24, 439.
- Chen, P. et al. (2019) Mol Brain 12, 74.
- Yuan, Z. et al. (2019) Cells 8, 552. doi: 10.3390/cells8060552.
- Zhang, T. et al. (2019) Glia 67, 688-702.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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