R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
NRMT (D9D6P) Rabbit mAb #13432
Filter:
- WB
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 25 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
NRMT (D9D6P) Rabbit mAb recognizes endogenous levels of total NRMT protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu49 of human NRMT protein.
Background
N-terminal RCC1 methyltransferase (NRMT), formerly known as methyltransferase-like protein 11A (METTL11A), is a member of the methyltransferase 11 family of proteins and is the first α-N-methyltransferase to be discovered in humans (1-3). Amino-terminal methylation of free α-amino groups is a post-translational modification where an initiating Met residue is cleaved and the exposed α–amino group is mono-, di-, or trimethylated by NRMT (4). NRMT methylates proteins containing an amino-terminal Met-X-Pro-Lys motif, where X is an alanine, proline, or serine residue (4). Substrates of NRMT include the Ran guanine nucleotide-exchange factor (RCC1), SET/TAF-1/PHAP-II, retinoblastoma (Rb), and CENP-B (3-6). α-N-methylation of RCC1 is required for efficient binding to chromatin, securing normal bipolar spindle formation and chromosome segregation (3,5). α-N-methylation of CENP-B also appears to regulate CENP-B binding to centromeric DNA (6).
- Wrighton, K.H. (2010) Nat Rev Mol Cell Biol 11, 604-5.
- Webb, K.J. et al. (2010) Biochemistry 49, 5225-35.
- Tooley, C.E. et al. (2010) Nature 466, 1125-8.
- Petkowski, J.J. et al. (2012) Biochemistry 51, 5942-50.
- Chen, T. et al. (2007) Nat Cell Biol 9, 596-603.
- Dai, X. et al. (2013) J Proteome Res 12, 4167-75.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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