Notch1 (5B5) Rat mAb (BSA and Azide Free) #31289
Filter:
- WB
- ELISA
Supporting Data
| REACTIVITY | H M R B |
| SENSITIVITY | Endogenous |
| MW (kDa) | 120, 300 |
| Source/Isotype | Rat IgG2b |
Application Key:
- WB-Western Blotting
- ELISA-ELISA
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- B-Bovine
Product Information
Product Usage Information
This product is the carrier free version of product #3447. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.
For standard formulation of this product see product #3447
For standard formulation of this product see product #3447
Storage
Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.
Specificity / Sensitivity
Notch1 (5B5) Rat mAb (BSA and Azide Free) detects endogenous levels of total Notch1 protein. It recognizes both the full-length (~ 300 kDa) protein and the transmembrane/intracellular region NTM (~120 kDa), which consists of a short extracellular juxtamembrane peptide, a transmembrane sequence and the intracellular domain (NICD). The antibody cannot detect the extracellular (ligand-binding) domain of Notch1 following cleavage at the S2 site by ADAM-type metalloproteases.
Species Reactivity:
Human, Mouse, Rat, Bovine
Source / Purification
Monoclonal antibody is produced by immunizing animals with a fusion protein corresponding to intracellular residues of Notch1.
Background
Neuro-oncological ventral antigen (NOVA) 1 and 2 are neuronal RNA-binding proteins (RBPs) that bind YCAY motifs to regulate alternative splicing (1-3). Both proteins have been shown to be targets in paraneoplastic opsoclonus myoclonus ataxia, a neuronal autoimmune disease (1,3). NOVA proteins rely on their KH3 domain to bind RNA and are reciprocally expressed in various parts of the brain (4). NOVA1 has been shown to be essential for neuronal viability, as it controls alternative splicing in GlyRalpha2 and GABA(A) receptor pre-mRNAs (5). NOVA1 is also capable of auto-regulation as it binds to its own exon 4, which contains a phosphorylated protein domain (6). NOVA1/2 double knockout mice are paralyzed due to lack of alternatively spliced agrin Z(+), which helps synapse formation at neuromuscular junctions (7). NOVA2 has been shown to regulate circular RNA biogenesis in the developing brain (8). NOVA1 has been shown to be highly expressed in many different cancer types, and can promote epithelial-mesenchymal transition by binding β-catenin mRNA (9,10).
- Buckanovich, R.J. et al. (1996) J Neurosci 16, 1114-22.
- Buckanovich, R.J. and Darnell, R.B. (1997) Mol Cell Biol 17, 3194-201.
- Schroeter, E.H. et al. (1998) Nature 393, 382-6.
- Lewis, H.A. et al. (1999) Structure 7, 191-203.
- Dredge, B.K. et al. (2005) EMBO J 24, 1608-20.
- Ruggiu, M. et al. (2009) Proc Natl Acad Sci U S A 106, 3513-8.
- Cao, Y. et al. (2021) Mol Cell Biochem 476, 279-292.
- Knupp, D. et al. (2021) Nucleic Acids Res 49, 6849-6862.
- Liu, M. et al. (2020) Transl Cancer Res 9, 4373-4382.
- Tang, S. et al. (2020) RNA Biol 17, 881-891.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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