Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP^® Rabbit mAb #19807

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Supporting Data

REACTIVITY	H M R Mk Z Dg
SENSITIVITY	Endogenous
MW (kDa)	92
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IP-Immunoprecipitation
IHC-Immunohistochemistry
IF-Immunofluorescence
F-Flow Cytometry

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat
Mk-Monkey
Z-Zebrafish
Dg-Dog

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50
Immunohistochemistry (Paraffin)	1:500 - 1:2000
Immunofluorescence (Frozen)	1:1600 - 1:6400
Immunofluorescence (Immunocytochemistry)	1:1600 - 1:6400
Flow Cytometry (Fixed/Permeabilized)	1:800 - 1:3200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #24710.

Protocol

Specificity / Sensitivity

Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP^® Rabbit mAb recognizes endogenous levels of β-catenin protein only when Ser45 is not phosphorylated.

Species Reactivity:

Human, Mouse, Rat, Monkey, Zebrafish, Dog

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser45 of human β-catenin protein.

Background

β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).

Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP^® Rabbit mAb specifically recognizes β-catenin that is non-phosphorylated at Ser45. Non-phospho β-Catenin (Ser45) protein is useful as a readout of stabilized β-catenin protein, and therefore functionally active in cell-cell adhesion and mediating transcriptional activity via the canonical Wnt signaling pathway (9).

Database Links

UniProt ID: P35222

Entrez-Gene Id: 1499

限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb #19807