R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
NMNAT1 (D7O4N) Rabbit mAb #98354
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 29 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
NMNAT1 (D7O4N) Rabbit mAb recognizes endogenous levels of total NMNAT1 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala268 of human NMNAT1 protein.
Background
Nicotinamide mononucleotide adenylyl transferases (NMNATs) catalyze the reversible reaction of ATP with NaMN (nicotinic acid mononucleotide) or NMN (nicotinamide mononucleotide) to produce NaAD (nicotinic acid adenine dinucleotide) or NAD (nicotinamide adenine dinucleotide). NAD is an essential cofactor or substrates for many enzymes like PARP1 and Sirt1 that regulate diverse cellular processes including oxidative reactions and transcription. NMNATs maintain NAD levels for internal homeostasis (1,2). NMNAT1 is localized to the nucleus and loss-of-function mutant in mice causes embryonic lethality (3). In humans, several different NMNAT1 mutations are associated with Leber congenital amaurosis (LCA), the most common cause of inherited childhood blindness (4-7).
- Jayaram, H.N. et al. (2011) Curr Med Chem 18, 1962-72.
- Ali, Y.O. et al. (2013) Trends Neurosci 36, 632-40.
- Conforti, L. et al. (2011) FEBS J 278, 2666-79.
- Chiang, P.W. et al. (2012) Nat Genet 44, 972-4.
- Koenekoop, R.K. et al. (2012) Nat Genet 44, 1035-9.
- Perrault, I. et al. (2012) Nat Genet 44, 975-7.
- Falk, M.J. et al. (2012) Nat Genet 44, 1040-5.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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