R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
NLRP12 (E8A3C) Rabbit mAb #39296
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 100-110 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
NLRP12 (E8A3C) Rabbit mAb recognizes endogenous levels of total NLRP12 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln190 of human NLRP12 protein.
Background
NLR family pyrin domain containing 12 (NLRP12, NALP12, PYPAF7, MONARCH-1), a member of the NOD-like receptor (NLR) family, is an intracellular protein that plays a role in forming an inflammasome and can also act as a regulator of inflammatory signaling (1). NLRP12 is expressed in dendritic cells, neutrophils, granulocytes, and macrophages (2). Like other NLR family members, NLRP12 is composed of a pyrin domain (PYD), a nucleotide-binding domain (NBD), and a leucine-rich repeat (LRR) domain (2). NLRP12 functions as a negative regulator of canonical NFκB signaling by inhibiting accumulation of hyperphosphorylated IRAK1 downstream of TLR signaling (3). NLRP12 also acts as a negative regulator of non-canonical NFκB signaling by associating with NFκB-inducing kinase (NIK) and TRAF3, which leads to proteasomal degradation of NIK (4, 5). During Yersinia pestis infection, NLRP12 acts as an inflammasome component, resulting in the release of IL-1β and IL-18 (6). NLRP12 plays a role in regulating gut microbiota, reducing susceptibility to colitis (7).
- Tuladhar, S. and Kanneganti, T.D. (2020) Mol Aspects Med 76, 100887.
- Williams, K.L. et al. (2003) J Immunol 170, 5354-8.
- Williams, K.L. et al. (2005) J Biol Chem 280, 39914-24.
- Lich, J.D. et al. (2007) J Immunol 178, 1256-60.
- Allen, I.C. et al. (2012) Immunity 36, 742-54.
- Vladimer, G.I. et al. (2012) Immunity 37, 96-107.
- Chen, L. et al. (2017) Nat Immunol 18, 541-551.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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