R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
NLRP10 (E5X5A) Rabbit mAb #61179
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 75 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
NLRP10 (E5X5A) Rabbit mAb recognizes endogenous levels of total NLRP10 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile340 of human NLRP10 protein.
Background
The nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) family of proteins is a diverse family of cytoplasmic innate immune receptors. They are characterized by the presence of an amino-terminal effector domain, which is often either a caspase activation and recruitment domain (CARD) or a pyrin domain (PYD), followed by a NACHT domain and carboxy-terminal leucine-rich-repeats (LRR) involved in recognition of pathogen-associated molecular patterns (PAMPs) (1). NLR proteins play a variety of roles during the innate immune response including pathogen sensing, transcriptional activation of proinflammatory cytokines through NF-κB, transcriptional activation of type I interferons through IRFs, and formation of inflammasomes leading to activation of inflammatory caspases (1-7).
NLRP10 (NLR family pyrin domain-containing protein 10, PYNOD, NALP10) is the only NLR family member lacking the characteristic LRR repeats (8-12). In response to a variety of stimuli, including mitochondrial damage, the NLRP10 inflammasome is formed, leading to ASC speck formation and caspase-1-dependent cytokine release (12).
NLRP10 (NLR family pyrin domain-containing protein 10, PYNOD, NALP10) is the only NLR family member lacking the characteristic LRR repeats (8-12). In response to a variety of stimuli, including mitochondrial damage, the NLRP10 inflammasome is formed, leading to ASC speck formation and caspase-1-dependent cytokine release (12).
- Elinav, E. et al. (2011) Immunity 34, 665-79.
- Inohara, N. et al. (1999) J Biol Chem 274, 14560-7.
- Ogura, Y. et al. (2001) J Biol Chem 276, 4812-8.
- Sabbah, A. et al. (2009) Nat Immunol 10, 1073-80.
- Mariathasan, S. et al. (2004) Nature 430, 213-8.
- Agostini, L. et al. (2004) Immunity 20, 319-25.
- Martinon, F. et al. (2002) Mol Cell 10, 417-26.
- Wang, Y. et al. (2004) Int Immunol 16, 777-86.
- Imamura, R. et al. (2010) J Immunol 184, 5874-84.
- Eisenbarth, S.C. et al. (2016) Nature 530, 504.
- Zheng, D. et al. (2023) Nat Immunol 24, 585-594.
- Próchnicki, T. et al. (2023) Nat Immunol 24, 595-603.
限制使用
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