R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
NLRC5 (D9Z5X) Rabbit mAb #33343
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 205 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
NLRC5 (D9Z5X) Rabbit mAb recognizes endogenous levels of total NLRC5 protein. This antibody recognizes isoforms 1-6 of NLRC5 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro131 of human NLRC5 protein.
Background
NLRC5 (NOD-like receptor family CARD domain containing 5, NOD4, CITA, NOD27) is a member of the NLR family of pattern recognition receptors (PRRs) involved in innate immunity (1,2). NLRC5 is predominantly expressed in hematopoietic cells, and is upregulated by IFN-γ (1-4). NLRC5 transcriptionally regulates the MHC class I antigen presentation pathway, and induces the expression of β2-microglobulin, TAP1, and LMP2 genes, in addition to MHC class I (3-5). In cancer, NLRC5 is critical for inducing cytotoxic CD8+ T cell activity and is a potential target for cancer immune evasion (6,7).
- Kuenzel, S. et al. (2010) J Immunol 184, 1990-2000.
- Cui, J. et al. (2010) Cell 141, 483-96.
- Meissner, T.B. et al. (2010) Proc Natl Acad Sci USA 107, 13794-9.
- Meissner, T.B. et al. (2012) Microbes Infect 14, 477-84.
- Meissner, T.B. et al. (2012) J Immunol 188, 4951-8.
- Yoshihama, S. et al. (2016) Proc Natl Acad Sci USA 113, 5999-6004.
- Shukla, A. et al. (2021) Int J Mol Sci 22, 1964. doi: 10.3390/ijms22041964.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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