R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
NKX2.5 (E1Y8H) Rabbit mAb #8792
Filter:
- WB
- IF
- F
Western blot analysis of extracts from various cell lines using NKX2.5 (E1Y8H) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 30-42 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- Related Products
- Conjugates
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:400 - 1:1600 |
| Flow Cytometry (Fixed/Permeabilized) | 1:800 - 1:1600 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #60876.
For a carrier free (BSA and azide free) version of this product see product #60876.
Protocol
Specificity / Sensitivity
NKX2.5 (E1Y8H) Rabbit mAb recognizes endogenous levels of total NKX2.5 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro67 of human NKX2.5 protein.
Background
NKX2.5 is a member of the NKX homeobox transcription factor family. NKX2.5 plays an essential role in heart development and is among the earliest factors expressed in the cardiac lineage in developing embryos. Targeted disruption of the murine Nkx2.5 gene results in abnormal heart morphogenesis, severe growth retardation, and embryonic lethality around E9.5 (1,2). Mutations in NKX2.5 are likewise associated with several congenital heart conditions, such as atrial defect with atrioventricular conduction defects (ASD-AVCD) and Tetralogy of Fallot (TOF) (3,4). Transcriptional activation of NKX2.5 is also associated with some B and T cell leukemias that result from chromosomal translocation (5-8).
- Lyons, I. et al. (1995) Genes Dev 9, 1654-66.
- Tanaka, M. et al. (1999) Development 126, 1269-80.
- Benson, D.W. et al. (1999) J Clin Invest 104, 1567-73.
- Reamon-Buettner, S.M. and Borlak, J. (2010) Hum Mutat 31, 1185-94.
- Nagel, S. et al. (2003) Cancer Res 63, 5329-34.
- Przybylski, G.K. et al. (2006) Haematologica 91, 317-21.
- Nagel, S. et al. (2007) Cancer Res 67, 1461-71.
- Su, X. et al. (2008) Haematologica 93, 1081-5.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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