R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
NKG7 (E6S2A) Rabbit mAb #84835
Filter:
- WB
- IF
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 7-25 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:200 |
| Flow Cytometry (Fixed/Permeabilized) | 1:50 - 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
NKG7 (E6S2A) Rabbit mAb recognizes endogenous levels of total NKG7 protein. This antibody may recognize multiple predicted isoforms of NKG7 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly161 of human NKG7 protein.
Background
Natural killer cell granule protein 7 (NKG7, also known as GMP-17 and GIG-I) is a novel type 1 integral membrane protein expressed at high levels in lymphocytes, including natural killer (NK) cells, γδ T cells, a subset of αβ T cells, lymphokine-activated killer (LAK) cells (1), and activated T regulatory cells (2). NKG7 expression can be induced in neutrophils following GM-CSF treatment (3). NKG7 protein is localized to the membranes of cytotoxic granules. Following target cell-induced NK cell degranulation, NKG7 translocates from granules to the plasma membrane (4). Studies have also shown that NKG7 is significantly upregulated in allograft rejection patients (5-8).
- Turman, M.A. et al. (1993) Hum Immunol 36, 34-40.
- Sugimoto, N. et al. (2006) Int Immunol 18, 1197-209.
- Shimane, M. et al. (1994) Biochem Biophys Res Commun 199, 26-32.
- Medley, Q.G. et al. (1996) Proc Natl Acad Sci U S A 93, 685-9.
- Sacreas, A. et al. (2018) PLoS One 13, e0205107.
- Sigdel, T.K. et al. (2015) PLoS One 10, e0138133.
- Ashokkumar, C. et al. (2011) Am J Pathol 179, 1929-38.
- Meehan, S.M. et al. (1997) Lab Invest 76, 639-49.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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