NKCC2 Antibody #38436
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 150-290 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
NKCC2 Antibody recognizes endogenous levels of total NKCC2 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe73 of human NKCC2 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The Na-K-2Cl cotransporter (NKCC2) is a sodium-potassium-chloride cotransporter. It is mainly expressed on the luminal membrane of renal epithelial cells of the thick ascending limb of Henle's loop (TALH) and mediates the majority of NaCl resorption and concentration of urine (1,2). NKCC2 is the target for several diuretic drugs, such as bumetanide, and is involved in the pathogenesis of hypertension (3,4). Mutations in the NKCC2-encoding gene, SLC12A1, causes Bartter’s syndrome, which is featured by impaired salt reabsorption in the TALH, hypokalemic metabolic alkalosis, and hypercalciuria (5,6). Recently, NKCC2 was reported to be expressed in the brain hypothalamo-neurohypophyseal system (HNS) and upregulated upon osmotic stress (7).
- Igarashi, P. et al. (1996) J Biol Chem 271, 9666-74.
- Kaplan, M.R. et al. (1996) J Clin Invest 98, 723-30.
- Markadieu, N. and Delpire, E. (2014) Pflugers Arch 466, 91-105.
- Orlov, S.N. et al. (2015) Genes Dis 2, 186-196.
- Vargas-Poussou, R. et al. (1998) Am J Hum Genet 62, 1332-40.
- Simon, D.B. et al. (1996) Nat Genet 13, 183-8.
- Konopacka, A. et al. (2015) J Neurosci 35, 5144-55.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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