R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
NID1/Nidogen-1 (E9J3L) Rabbit mAb #86513
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 150 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:50 - 1:250 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
NID1/Nidogen-1 (E9J3L) Rabbit mAb recognizes endogenous levels of total NID1/nidogen-1 protein. This antibody cross-reacts with a band of unknown origin at approximately 80 kDa.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1186 of human NID1/nidogen-1 protein.
Background
All extracellular basement membrane contains four major components: type IV collagen, nidogens, laminins, and perlecan (1). Nidogens are a family of highly conserved, sulfated glycoproteins which interact with the laminin 1 short arm chain, type IV collagen, and perlecan. This linking effect contributes to basement membrane formation (2,3). There are two isoforms in the nidogen family, nidogen-1, and nidogen-2. They have a similar domain structure consisting of three globular domains separated by a link region and a rod region (3). The functions of the two isoforms are complementary. Knockout of both genes results in defective basement membrane formation and perinatal lethality due to abnormal lung/heart/limb development (4,5). In lung cancer, colorectal cancer, and ovarian cancer, increased nidogen-1 release contributes to tumor progression by promoting proliferation and migration/invasion, EMT, and metastasis (6-10).
- Pozzi, A. et al. (2017) Matrix Biol 57-58, 1-11.
- Dziadek, M. (1995) Experientia 51, 901-13.
- Zhou, S. et al. (2022) Genes Dis 9, 598-609.
- Bader, B.L. et al. (2005) Mol Cell Biol 25, 6846-56.
- Böse, K. et al. (2006) J Biol Chem 281, 39620-9.
- Alečković, M. et al. (2017) Genes Dev 31, 1439-1455.
- Jagroop, R. et al. (2021) Oncol Lett 21, 52.
- Vaes, N. et al. (2021) EMBO Rep 22, e51913.
- Zhou, Y. et al. (2017) Oncotarget 8, 33110-33121.
- Rokavec, M. et al. (2019) Cell Mol Gastroenterol Hepatol 7, 783-802.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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