R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
NHE1 (E2S8S) Rabbit mAb #46386
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 80-110 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
NHE1 (E2S8S) Rabbit mAb recognizes endogenous levels of total NHE1 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human NHE1 protein.
Background
The sodium/hydrogen exchanger (NHE) family of proteins are membrane-bound antiporters that extrude extracellular Na+ in exchange for intracellular H+ (1). By exchanging protons for sodium ions, NHE plays an important role in diverse physiological processes, including cell cycle, size, and proliferation, vesicle tracking and biogenesis, and ion homeostasis. The mammalian genome contains 13 NHE family members encoded by the SLC9 gene family. NHE1, encoded by the SLC9A1 gene, has a complex structure consisting of 13 transmembrane regions (2,3). Dysfunction of NHE1 is implicated in tumor progression and is genetically linked to several neurological disorders, including Lichtenstein-Knorr syndrome (4,5).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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