R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
NGF (E7O4E) Rabbit mAb #90605
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Recombinant and Transfected |
| MW (kDa) | Precursor 28 - Mature 13 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
NGF (E7O4E) Rabbit mAb recognizes mouse recombinant NGF, and exogenously expressed NGF protein.
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse NGF protein.
Background
Nerve growth factor (NGF) is a small, secreted protein and member of the neurotrophin family of growth factors that promote neuronal cell survival and differentiation (1). Producing cells release NGF that bind and activate TrkA high affinity receptors to mediate NGF-driven signaling. NGF also binds to low affinity p75 (NTR) receptors, which belong to the death receptor family (2). Although NGF has been classically described as favoring neuron survival and differentiation, NGF can promote apoptosis in cells that contain p75 (NTR) and lack TrkA. NGF can induce neuron death in a variety of neurodegenerative conditions, including Alzheimer's disease (3). Besides its neurotrophic actions, NGF has an effect on non-neuronal cells and may help mediate inflammation, angiogenesis, and stimulate breast cancer cell growth (4-6). NGF signaling is looking increasingly promising as potential drug targets for diseases.
- Lessmann, V. et al. (2003) Prog Neurobiol 69, 341-74.
- Neet, K.E. and Campenot, R.B. (2001) Cell Mol Life Sci 58, 1021-35.
- Lorigados-Pedre, L. and Bergado-Rosado, J. (2004) Rev Neurol 38, 957-71.
- Frossard, N. et al. (2004) Eur J Pharmacol 500, 453-65.
- Nico, B. et al. (2008) Microvasc Res 75, 135-41.
- Descamps, S. et al. (2001) J Biol Chem 276, 17864-70.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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