R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
NFS1 (F1E7S) Rabbit mAb #99136
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 45 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:50 - 1:250 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
NFS1 (F1E7S) Rabbit mAb recognizes endogenous levels of total NFS1 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human NFS1 protein.
Background
Cysteine desulfurase (NFS1) is a component of the core complex of the de novo iron-sulfur cluster (ISC) assembly machinery in the mitochondrial matrix (1). This enzyme desulfurates L-cysteine. The sulfur from cysteine is used for the biosynthesis of ISCs (2,3). Studies show that high levels of NFS1 are essential to cancer cells when oxygen levels are above the 3–8% oxygen concentration typical of most tissues. Strong NFS1 expression is necessary for primary lung tumor growth and NFS1 protects lung adenocarcinomas from ferroptosis in reaction to oxidative damage (2). In addition, suppression of NFS1 expression leads to growth inhibition of some breast cancer cells (3).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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