R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
NFI-B (E5L7U) Rabbit mAb #24903
Filter:
- WB
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 50-75 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:1600 - 1:3200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
NFI-B (E5L7U) Rabbit mAb recognizes endogenous levels of total NFI-B protein. Based on antigen sequence, the antibody is predicted to detect isoform 1 and isoforms 4-6 of NFI-B. Based on sequence homology, this antibody is not predicted to cross-react with other NFI family members. Species cross-reactivity for IF-IC is human only.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro404 of human NFI-B protein.
Background
NFI-B belongs to the nuclear factor I (NFI) family of site-specific transcription factors that regulate viral DNA replication and expression of various genes (1,2). The NFI family is composed of four members in vertebrates: NFI-A, NFI-B, NFI-C, and NFI-X, all of which are critical in the development of multiple organ systems in mice and humans (3). Research studies have demonstrated that NFI-B plays a crucial role in different aspects of tumor development, acting as an oncogene or tumor suppressor in different types of tumors and participating in tumor-associated fusion gene formation (4-7). Moreover, NFI-B has been shown to promote epithelial-mesenchymal transition and metastatic ability of cancer cells through a number of mechanisms, including upregulating SNAI1 expression in colorectal cancer (8), stabilizing chromatin accessibility in small cell lung cancer (9), and modulating the ZEB1 axis in melanoma (10).
- Gronostajski, R.M. (2000) Gene 249, 31-45.
- Liang, B. et al. (2012) J Virol 86, 3264-75.
- Steele-Perkins, G. et al. (2003) Mol Cell Biol 23, 1075-84.
- Stringer, B.W. et al. (2016) Oncotarget 7, 29306-20.
- Moon, H.G. et al. (2011) Mol Oncol 5, 538-44.
- Wang, N. et al. (2021) PeerJ 9, e10848.
- Wagner, V.P. et al. (2022) Crit Rev Oncol Hematol 176, 103745.
- Liu, Z. et al. (2019) Cancer Sci 110, 86-98.
- Denny, S.K. et al. (2016) Cell 166, 328-342.
- Cheng, R. et al. (2021) Oncol Lett 21, 81.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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