Render Target: SSR
Render Timestamp:
4/2/2025, 6:18:57 PM EDT
4/2/2025, 10:18:57 PM UTC
Commit: 461ca8d8fe5b1efd4c01fc87e5b5eb592e2d154a
XML generation date: 2025-03-07 13:17:53.157
Product last modified at: 2025-03-08T08:01:44.582Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

NeuN (E4M5P) Mouse mAb (BSA and Azide Free) #93972

Filter:
  • WB
  • IF
Western Blotting Image 1: NeuN (E4M5P) Mouse mAb (BSA and Azide Free)
Western blot analysis of extracts from mouse and rat brain tissue using NeuN (E4M5P) Mouse mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Data were generated using the standard formulation of this product.

To Purchase # 93972

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 46-55
Source/Isotype Mouse IgG2a kappa
Application Key:
  • WB-Western Blotting 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Related Products

Product Information

Product Usage Information

This product is the carrier free version of product #94403. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

Formulation

Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

For standard formulation of this product see product #94403

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

NeuN (E4M5P) Mouse mAb (BSA and Azide Free) recognizes endogenous levels of total NeuN protein. This antibody also recognizes a non-specific band of unknown origin at 140 kDa.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human NeuN protein.

Background

Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, or dentate nucleus neurons (1). This neuronal protein was originally identified by immunoreactivity with a monoclonal antibody also called NeuN. Using MS analysis, NeuN was later identified as the Fox-3 gene product. Fox-3 contains an RNA recognition motif and functions as a splicing regulator (2). Fox-3 regulates alternative splicing of NumB, promoting neuronal differentiation during development (3).
For Research Use Only. Not For Use In Diagnostic Procedures.
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