R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Neogenin (D7M8E) Rabbit mAb #39447
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 210 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Neogenin (D7M8E) Rabbit mAb recognizes endogenous levels of total neogenin protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile1250 of human neogenin protein.
Background
Neogenin is a member of the Deleted in Colorectal Cancer (DCC) receptor family. It acts as an attractive axon guidance receptor in response to netrin, and as a repellent receptor when interacting with RGMa (1,2). Neogenin also regulates adhesion and tissue organization, such as bone formation, mammary gland morphogenesis, and skeletal myofiber development, by interacting with secreted netrins (3-5). In addition, neogenin is a key regulator of adult neurogenesis by synchronizing neuroblast migration and differentiation (6). Neogenin expression has been found to be associated with cellular phenotype in cancer. In breast cancer, neogenin expression is inversely correlated with cancer grade (7), while in gastric cancer, up regulation of neogenin was found to increase cell proliferation and motility (8).
- Rajagopalan, S. et al. (2004) Nat Cell Biol 6, 756-62.
- Wilson, B.D. et al. (2006) Science 313, 640-4.
- Kang, J.S. et al. (2004) J Cell Biol 167, 493-504.
- Srinivasan, K. et al. (2003) Dev Cell 4, 371-82.
- Hagihara, M. et al. (2011) J Biol Chem 286, 5157-65.
- O'Leary, C.J. et al. (2015) Stem Cells 33, 503-14.
- Xing, W. et al. (2014) World J Surg Oncol 12, 352.
- Kim, S.J. et al. (2014) Oncotarget 5, 3386-98.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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