渲染靶标:SSR
Render Timestamp: 2025-03-17T22:22:08.294Z
Commit: 9fc0f116116d9da247dc8ddd4e5fe811153412e1
XML generation date: 2024-09-30 01:59:32.681
Product last modified at: 2025-01-02T18:30:08.461Z
1% for the Planet 标识
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

NEK7 (E4E1B) Rabbit mAb #87795

Filter:
  • WB
  • IP
Western Blotting Image 1: NEK7 (E4E1B) Rabbit mAb
Western blot analysis of extracts from various cell lines using NEK7 (E4E1B) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Low expression of NEK7 protein in Hep G2 and mES cells is consistent with the predicted expression pattern.

To Purchase # 87795

Cat. #

Size

Price

Inventory

87795T
20 µl
¥1,500

无库存

87795S
100 µl
¥4,990

无库存

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 32
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Simple Western™ 1:10 - 1:50
Immunoprecipitation 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

NEK7 (E4E1B) Rabbit mAb recognizes endogenous levels of total NEK7 protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human NEK7 protein.

Background

NEK7 (NIMA-related protein kinase 7), is one of a family of serine/threonine kinases, named NEK1–NEK11, which are involved in the control of mitosis and other non-mitotic functions (1-6). NEK7 is the smallest NIMA-related kinase in mammals, and is widely expressed by many tissues (1-3). Its role in mitosis relates to centrosome enrichment and microtubule nucleation, and similar to other NIMA-related kinases, NEK7 is localized to the microtubule-organizing center (MTOC) at the time of mitotic entry (3,6). NEK7 has also been demonstrated to be essential for NLRP3 inflammasome activation by directly binding to the leucine-rich repeat (LRR) domain of NLRP3 (4-6).
For Research Use Only. Not For Use In Diagnostic Procedures.
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