Nectin-4/PVRL4 Antibody #17402
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 62, 75 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Nectin-4/PVRL4 Antibody recognizes endogenous levels of total Nectin-4/PVRL4 protein. Based upon sequence alignment, this antibody is not predicted to cross-react with PVR, PVRL1, PVRL2, and PVRL3 proteins.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Nectin-4/PVRL4 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Nectin-4/poliovirus receptor-related 4 (PVRL4) is a type-I transmembrane glycoprotein that belongs to the immunoglobulin superfamily and promotes cell-cell adhesion by serving as a major component of adherens junctions (1-3). The extracellular domain of Nectin-4, which contains an Ig variable-like domain (V) and two Ig constant-like domains (C), mediates binding to the measles virus (4) and to neighboring cells through trans heterophilic interactions with Nectin-1 (5,6). Unlike other nectin family members, which are widely expressed in adult tissues, Nectin-4 expression in humans is largely restricted to the placenta (7). Research studies have demonstrated that Nectin-4 is overexpressed in a variety of human solid tumors of the pancreas (8), breast (9,10), lung (11), and ovary (12). Due to its restricted expression pattern in normal human tissues, Nectin-4 may serve as a novel diagnostic and therapeutic target for a variety of human tumors.
- Takai, Y. et al. (2008) Annu Rev Cell Dev Biol 24, 309-42.
- Takai, Y. and Nakanishi, H. (2003) J Cell Sci 116, 17-27.
- Harrison, O.J. et al. (2012) Nat Struct Mol Biol 19, 906-15.
- Mühlebach, M.D. et al. (2011) Nature 480, 530-3.
- Mateo, M. et al. (2014) J Virol 88, 14161-71.
- Fabre, S. et al. (2002) J Biol Chem 277, 27006-13.
- Reymond, N. et al. (2001) J Biol Chem 276, 43205-15.
- Nishiwada, S. et al. (2015) J Exp Clin Cancer Res 34, 30.
- Lattanzio, R. et al. (2014) Oncogenesis 3, e118.
- Fabre-Lafay, S. et al. (2007) BMC Cancer 7, 73.
- Takano, A. et al. (2009) Cancer Res 69, 6694-703.
- Nabih, E.S. et al. (2014) Biomarkers 19, 498-504.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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