R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Nectin-2/CD112 (D8D3F) XP® Rabbit mAb #95333
Filter:
- WB
- IHC
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 70-80 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #51172.
For a carrier free (BSA and azide free) version of this product see product #51172.
Protocol
Specificity / Sensitivity
Nectin-2/CD112 (D8D3F) XP® Rabbit mAb recognizes endogenous levels of total Nectin-2/CD112 protein.
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human Nectin-2/CD112 protein.
Background
Nectin-2, also known as CD112 and poliovirus receptor-related 2 (PVRL2), is a single-pass type I membrane glycoprotein ubiquitously expressed on various human tissues (1). It is a calcium independent cell adhesion molecule known to interact with several cell surface receptors, including DNAM-1 (CD226), LFA-1 (CD11a), Nectin-3 (CD113), TIGIT (VSTM3), and PVRIG (CD112R) (2-7). It is one of the major constituents of adherens junctions, and therefore plays a central role in a number of cellular processes, including adhesion, migration, and proliferation (2-8). Within the immune system, Nectin-2 modulates immune cell signaling. Upon interaction with DNAM-1 expressed on T and NK cells, Nectin-2 stimulates proliferation and cytokine production (4). Upon interaction with PVRIG, Nectin-2 inhibits proliferation (7). Thus, Nectin-2 can be either a co-stimulator or a co-inhibitor of immune cell function depending on competitive receptor interactions. Nectin-2 also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses (8,9). Alternate transcriptional splice variants, encoding different isoforms, have been characterized (10-12).
- Eberlé, F. et al. (1995) Gene 159, 267-72.
- Lopez, M. et al. (1998) Blood 92, 4602-11.
- Satoh-Horikawa, K. et al. (2000) J Biol Chem 275, 10291-9.
- Bottino, C. et al. (2003) J Exp Med 198, 557-67.
- Reymond, N. et al. (2004) J Exp Med 199, 1331-41.
- Deuss, F.A. et al. (2017) J Biol Chem 292, 11413-22.
- Zhu, Y. et al. (2016) J Exp Med 213, 167-76.
- Devilard, E. et al. (2013) PLoS One 8, e77424.
- Martinez, W.M. and Spear, P.G. (2001) J Virol 75, 11185-95.
- Warner, M.S. et al. (1998) Virology 246, 179-89.
- Ota, T. et al. (2004) Nat Genet 36, 40-5.
- Gerhard, D.S. et al. (2004) Genome Res 14, 2121-7.
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
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For Research Use Only. Not For Use In Diagnostic Procedures.
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