R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
NCR1/NKp46 (E2K4N) Rabbit mAb #23732
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 50-60 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
NCR1/NKp46 (E2K4N) Rabbit mAb recognizes endogenous levels of total NCR1/NKp46 protein.
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse NCR1/NKp46 protein.
Background
The family of natural cytotoxicity receptors (NCRs) contains NCR1 (NKp46/CD335), NCR2 (NKp44/CD336), and NCR3 (NKp30/CD337). They are single-pass type I transmembrane proteins belonging to the immunoglobin (Ig) superfamily. Various pathogen-derived as well as host-encoded molecules have been identified as ligands for the NCRs. They were originally identified by their ability to evoke the cytotoxicity of natural killer (NK) cells toward tumor cells in vitro, and animal models have demonstrated that NCRs play a role in vivo in tumor surveillance, viral infection, and pregnancy (1-5).
NCR1/NKp46 is considered a universal marker for NK cells, and recent studies found that it is also expressed by other cells, such as group 1 innate lymphoid cells (ILC1), a subset of group 3 ILCs (NCR+ ILC3), and γδ T cells (2-4). NCR1/NKp46 is also expressed on and considered as a diagnostic marker and therapeutic target for some malignant NK, NKT, and T cell lymphomas (6,7). Cross-linking NCR1/NKp46 with antibodies can activate NK cells and this has been pursued as a promising therapeutic avenue (8).
NCR1/NKp46 is considered a universal marker for NK cells, and recent studies found that it is also expressed by other cells, such as group 1 innate lymphoid cells (ILC1), a subset of group 3 ILCs (NCR+ ILC3), and γδ T cells (2-4). NCR1/NKp46 is also expressed on and considered as a diagnostic marker and therapeutic target for some malignant NK, NKT, and T cell lymphomas (6,7). Cross-linking NCR1/NKp46 with antibodies can activate NK cells and this has been pursued as a promising therapeutic avenue (8).
- Sivori, S. et al. (1997) J Exp Med 186, 1129-36.
- Kruse, P.H. et al. (2014) Immunol Cell Biol 92, 221-9.
- Pazina, T. et al. (2017) Front Immunol 8, 369.
- Barrow, A.D. et al. (2019) Front Immunol 10, 909.
- Meza Guzman, L.G. et al. (2020) Cancers (Basel) 12, 952.
- Laribi, K. et al. (2018) Recent Pat Anticancer Drug Discov 13, 308-40.
- Cheminant, M. et al. (2019) Gut 68, 1396-405.
- Gauthier, L. et al. (2019) Cell 177, 1701-1713.e16.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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