R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
NCOA4 (E8H8Z) Rabbit mAb #66849
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 80 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
NCOA4 (E8H8Z) Rabbit mAb recognizes endogenous levels of total NCOA4 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly213 of human NCOA4 protein.
Background
Nuclear Receptor Coactivator 4 (NCOA4), also known as ARA70 or ELE1, was initially discovered as a transcriptional coactivator of androgen receptor (1,2). NCOA4 has also been implicated in regulation of peroxisome proliferator-activated receptor gamma and alpha, as well as estrogen receptor alpha (3-5). NCOA4 plays an autophagic role as a cargo receptor by shuttling ferritin to the lysosome in order to maintain iron homeostasis, which ultimately controls heme synthesis and red blood cell development (6-9). The NCOA4 gene has been shown to undergo a fusion event with the RET proto-oncogene, forming RET/PTC3 in thyroid and other types of cancers (10-13).
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- Miyamoto, H. et al. (1998) Proc Natl Acad Sci U S A 95, 7379-84.
- Heinlein, C.A. et al. (1999) J Biol Chem 274, 16147-52.
- Lanzino, M. et al. (2005) J Biol Chem 280, 20421-30.
- Heinlein, C.A. and Chang, C. (2003) Endocrine 21, 139-46.
- Mancias, J.D. et al. (2014) Nature 509, 105-9.
- Dowdle, W.E. et al. (2014) Nat Cell Biol 16, 1069-79.
- Mancias, J.D. et al. (2015) Elife 4, doi: 10.7554/eLife.10308.
- Ryu, M.S. et al. (2017) J Clin Invest 127, 1786-97.
- Santoro, M. et al. (1994) Oncogene 9, 509-16.
- Minoletti, F. et al. (1994) Genes Chromosomes Cancer 11, 51-7.
- Le Rolle, A.F. et al. (2015) Oncotarget 6, 28929-37.
- Weinreb, I. et al. (2018) Am J Surg Pathol 42, 442-52.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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