R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
NBR1 (E6Q3F) Rabbit mAb #20145
Filter:
- WB
- IP
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 140 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:400 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
NBR1 (E6Q3F) Rabbit mAb recognizes endogenous levels of total NBR1 protein. This antibody is suitable for detecting NBR1 by immunofluorescence when it's expressed at moderate-to-high levels.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro597 of human NBR1 protein.
Background
Next to BRCA1 gene 1 (NBR1) protein is known for its encoding gene proximity to the BRCA1 tumor suppressor gene (1,2). N-terminal Phox and Bem1p (PB1) domains of NBR1 mediate its interaction with muscle specific titin kinase (3,4) and scaffolding protein p62 (4). NBR1 plays a role in selective autophagy by facilitating the autophagosomal degradation of ubiquitinated proteins independently and also in concert with p62 (5-11).
- Campbell, I.G. et al. (1994) Hum Mol Genet 3, 589-94.
- Miki, Y. et al. (1994) Science 266, 66-71.
- Lange, S. et al. (2005) Science 308, 1599-603.
- Müller, S. et al. (2006) FEBS Lett 580, 341-4.
- Kirkin, V. et al. (2009) Mol Cell 33, 505-16.
- Lamark, T. et al. (2009) Cell Cycle 8, 1986-90.
- Kenific, C.M. and Debnath, J. (2016) Autophagy 12, 1958-1959.
- Hafrén, A. and Hofius, D. (2017) Autophagy 13, 2000-2001.
- Werner, A. et al. (2019) Autophagy 15, 78-97.
- Sánchez-Martín, P. et al. (2020) EMBO Rep 21, e48902.
- Turco, E. et al. (2021) Nat Commun 12, 5212.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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