R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
NAT10 (E5U9W) Rabbit mAb #30413
Filter:
- WB
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 120 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
NAT10 (E5U9W) Rabbit mAb recognizes endogenous levels of total NAT10 protein.
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu228 of human NAT10 protein.
Background
N-acetyltransferase 10 (NAT10), also known as human N-acetyltransferase-like protein (hALP), is an acetyltransferase that increases telomerase activity through the activation of the TERT promoter (1). NAT10 localizes to the midbody in late phase mitosis to help achieve cytokinesis (2). It has been found that DNA damage can actually increase NAT10-dependent acetylation of alpha-tubulin, resulting in increased stability of the protein (2,3). This increased stability of microtubules has been linked to the sequestration of the nuclear import factor transportin-1 in the premature aging disease, progeria (4,5). NAT10 can also function as an acetyltransferase for both rRNA and mRNA (6-8). The acetylation of the 18 S rRNA helps in rRNA processing and ribosome biogenesis, and this process can be disrupted by opposing activity of the deacetylase SirT1 in response to stress (6,9). mRNA acetylation by NAT10 plays a role in translation efficiency and mRNA stability (7,8). NAT10 is overexpressed in many different cancer types, making it a good potential therapeutic target (10-12).
- Lv, J. et al. (2003) Biochem Biophys Res Commun 311, 506-13.
- Shen, Q. et al. (2009) Exp Cell Res 315, 1653-67.
- Liu, H. et al. (2007) Mol Cell Biochem 300, 249-58.
- Larrieu, D. et al. (2018) Sci Signal 11, eaar5401. doi: 10.1126/scisignal.aar5401.
- Wilson, K.L. (2018) Sci Signal 11, eaat9448. doi: 10.1126/scisignal.aat9448.
- Ito, S. et al. (2014) J Biol Chem 289, 35724-30.
- Arango, D. et al. (2018) Cell 175, 1872-1886.e24.
- Dominissini, D. and Rechavi, G. (2018) Cell 175, 1725-1727.
- Liu, X. et al. (2018) Nucleic Acids Res 46, 9601-9616.
- Liang, P. et al. (2020) Curr Probl Cancer 44, 100491.
- Zi, J. et al. (2020) Front Oncol 10, 598107.
- Liu, H.Y. et al. (2020) Nucleic Acids Res 48, 3638-3656.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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