Naked2 (C36D3) Rabbit mAb #2596
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Transfected Only |
| MW (kDa) | 59, 61 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Naked2 (C36D3) Rabbit mAb detects transfected levels of mouse Naked2.
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly47 of mouse Naked2.
Background
Naked1 (Nkd1) and Naked2 (Nkd2) are homologs of Drosophila Naked cuticle, a negative regulator of Wnt/Wingless signaling pathway which functions through a feedback mechanism (1,2). Both Drosophila and vertebrate Naked proteins contain a putative calcium-binding EF-hand motif, however, Drosophila Naked binds to zinc instead of calcium (3). Naked inhibits the canonical Wnt/β-catenin pathway by binding to Dishevelled proteins and directs Dishevelled activity towards the planar cell polarity pathway (2,4). Naked1 is a direct target of Wnt signaling and is overexpressed in some colon tumors due to constitutive activation of Wnt/β-catenin pathway (5). Naked2 is myristoylated and is required for sorting of TGF-α to the basolateral plasma membrane of polarized epithelial cells (6).
- Zeng, W. et al. (2000) Nature 403, 789-795.
- Wharton, K.A. et al. (2001) Dev. Biol. 234, 93-106.
- Rousset, R. et al. (2002) J. Biol. Chem. 277, 49019-49026.
- Yan, D. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 3802-3807.
- Yan, D. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 14973-14978.
- Li, C. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 5571-5576.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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