R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
MYH7 (E3G8Q) Rabbit mAb #64038
Filter:
- WB
Supporting Data
| REACTIVITY | H R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 140-210 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
MYH7 (E3G8Q) Rabbit mAb recognizes endogenous levels of total MYH7 protein.
Species Reactivity:
Human, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu1932 of human MYH7 protein.
Background
Myosin heavy chain 7 (MYH7), also known as β-myosin heavy chain, is a type I slow-twitch fiber expressed in muscle fibers as well as heart ventricles and is essential for muscle contraction (1). MYH7 is composed of a globular head domain at the N-terminus, a hinge region, and an α-helical coil-coil rod domain (2). MYH7 forms a hexameric complex with two MYH7, two myosin light chain 2 (MYL2), and two myosin light chain 3 (MYL3) (3). The myosin-binding sites on actin filaments become exposed by the binding of Ca+, which leads to actin-myosin junction formation and the ability to glide along the actin base. The complexes become detached in the presence of ATP, and the next cycle of contraction is initiated when ATPase activity of the head domain hydrolyzes ATP (4). Mutations in the MYH7 gene can lead to hypertrophic cardiomyopathy (HCM), dilated CM (DCM), and left ventricle non-compaction (LVNC) (5).
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
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For Research Use Only. Not For Use In Diagnostic Procedures.
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