MURF1 Antibody #4305
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Inquiry Info. # 4305
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Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 41 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
MURF1 Antibody detects endogenous levels of total MURF1 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MURF1 protein. Antibodies were purified by protein A and peptide affinity chromatography.
Background
Muscle-specific RING finger protein 1 (MURF1) is an E3 ubiquitin ligase that promotes muscle cell protein degradation during muscle atrophy (1). In response to conditions that promote muscle atrophy, elevated skeletal muscle MURF1 facilitates the ubiquitination and degradation of thick filaments components (2). Cardiac muscle MURF1 promotes degradation of cardiomyocyte troponin I and inhibits PKCε-mediated cardiac hypertrophy (3,4) MURF1 also regulates the degradation of the large sarcomeric protein titin, the transcriptional regulator GMEB1, the SUMO conjugating enzyme UBC9, and the ubiquitin peptidase USP13 (5). Binding of FoxO1 and the glucocorticoid receptor to adjacent sites within the MURF1 promoter region activates transcription of this E3 ubiquitin ligase (6).
- Bodine, S.C. et al. (2001) Science 294, 1704-8.
- Cohen, S. et al. (2009) J Cell Biol 185, 1083-95.
- Kedar, V. et al. (2004) Proc Natl Acad Sci USA 101, 18135-40.
- Arya, R. et al. (2004) J Cell Biol 167, 1147-59.
- McElhinny, A.S. et al. (2002) J Cell Biol 157, 125-36.
- Waddell, D.S. et al. (2008) Am J Physiol Endocrinol Metab 295, E785-97.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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