R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
MUC1-C (D5K9I) XP® Rabbit mAb (BSA and Azide Free) #50160
Filter:
- WB
- IHC
- IF
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 25 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
This product is the carrier free version of product #16564. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.
For standard formulation of this product see product #16564
For standard formulation of this product see product #16564
Storage
Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.
Specificity / Sensitivity
MUC1-C (D5K9I) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total MUC1-C protein. This antibody does not detect MUC1-N protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly1192 of human MUC1 protein.
Background
Mucins represent a family of glycoproteins characterized by repeat domains and dense O-glycosylation (1). MUC1 (or mucin 1) is aberrantly overexpressed in most human carcinomas. Increased expression of MUC1 in carcinomas reduces cell-cell and cell-ECM interactions. MUC1 is cleaved proteolytically, and the large ectodomain can remain associated with the small 25 kDa carboxy-terminal domain that contains a transmembrane segment and a 72-residue cytoplasmic tail (1). MUC1 interacts with ErbB family receptors and potentiates ERK1/2 activation (2). MUC1 also interacts with β-catenin, which is regulated by GSK-3β, PKCγ, and Src through phosphorylation at Ser44, Thr41, and Tyr46 of the MUC1 cytoplasmic tail (3-5). Overexpression of MUC1 potentiates transformation (6) and attenuates stress-induced apoptosis through the Akt or p53 pathways (7,8).
MUC1-C is the carboxy-terminal transmembrane subunit of MUC1 resulting from proteolytic cleavage of the full length protein. MUC1-N is the amino-terminal subunit, which can be tethered to MUC1-C, or released from the plasma membrane. MUC1-C interacts with receptor tyrosine kinases, β-catenin and other signaling proteins, and is thought to induce activation of MAPK, Akt and Wnt pathways. Due to its signaling functions and expression in human cancer, MUC1-C is a potential therapeutic target (reviewed in 9).
MUC1-C is the carboxy-terminal transmembrane subunit of MUC1 resulting from proteolytic cleavage of the full length protein. MUC1-N is the amino-terminal subunit, which can be tethered to MUC1-C, or released from the plasma membrane. MUC1-C interacts with receptor tyrosine kinases, β-catenin and other signaling proteins, and is thought to induce activation of MAPK, Akt and Wnt pathways. Due to its signaling functions and expression in human cancer, MUC1-C is a potential therapeutic target (reviewed in 9).
- Baldus, S.E. et al. (2004) Crit Rev Clin Lab Sci 41, 189-231.
- Schroeder, J.A. et al. (2001) J Biol Chem 276, 13057-64.
- Li, Y. et al. (1998) Mol Cell Biol 18, 7216-24.
- Li, Y. et al. (2001) J Biol Chem 276, 6061-4.
- Ren, J. et al. (2002) J Biol Chem 277, 17616-22.
- Schroeder, J.A. et al. (2004) Oncogene 23, 5739-47.
- Raina, D. et al. (2004) J Biol Chem 279, 20607-12.
- Wei, X. et al. (2005) Cancer Cell 7, 167-78.
- Kufe, D.W. (2013) Oncogene 32, 1073-81.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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