R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
MTF2 (F2X1A) Rabbit mAb #46454
Filter:
- WB
- IP
- ChIP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 50, 55, 70 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
For optimal ChIP results, use 2.5 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Chromatin IP | 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
MTF2 (F2X1A) Rabbit mAb recognizes endogenous levels of total MTF2 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly521 of human MTF2 protein.
Background
Metal regulatory transcription factor 2 (MTF2), also referred to as polycomb-like 2 (PCL2), is a catalytically inactive member of the PCL protein family (1). MTF2 binds to the metal response element of the mouse metallothionein promoter (2,3) and has also been shown to regulate the PRC2 activity at specific developmental genes in embryonic stem cells (4,5). It was also demonstrated that MTF2 promotes the proliferation and invasion of hepatocellular carcinoma cells (6) and that the MTF2–MDM2 axis can be targeted to sensitize refractory acute myeloid leukemia (AML) to chemotherapy (7).
- Walker, E. et al. (2010) Cell Stem Cell 6, 153-66.
- Casanova, M. et al. (2011) Development 138, 1471-82.
- Inouye, C. et al. (1994) DNA Cell Biol 13, 731-42.
- Perino, M. et al. (2018) Nat Genet 50, 1002-1010.
- Walker, E. et al. (2011) Cell Cycle 10, 45-51.
- Wu, T.T. et al. (2019) Onco Targets Ther 12, 11207-11220.
- Maganti, H.B. et al. (2018) Cancer Discov 8, 1376-1389.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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