R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
MTA3 (E3X2E) Rabbit mAb #37489
Filter:
- WB
- IP
- ChIP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 62, 66 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
For optimal ChIP results, use 5 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Chromatin IP | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
MTA3 (E3X2E) Rabbit mAb recognizes endogenous levels of total MTA3 protein. This antibody does not recognize MTA1 or MTA2 protein. This antibody detects a 200 kDa protein of unknown origin.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val438 of human MTA3 protein.
Background
Metastasis associated gene 3 (MTA3) is a cell-type specific subunit of the NuRD deacetylase complex. Much like MTA1 and MTA2, MTA3 does not associate with other family members, forming a unique NuRD complex. MTA3 has been shown to be estrogen-dependent and a key regulator of Snail and E-cadherin in breast cancer (1). B lymphocyte differentiation has been shown to be dependent on the ability of BCL6 to use MTA3 as a cofactor (2). MTA3 has also been shown to repress the Wnt4 pathway (3). MTA3 activity has been described in many different cancer types, including breast, non-small cell lung cancer, and B-cell lymphomas (1,4,5).
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
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For Research Use Only. Not For Use In Diagnostic Procedures.
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