R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
MS4A4A (E7J5D) Rabbit mAb #29717
Filter:
- WB
- IP
Western blot analysis of extracts from various cell lines using MS4A4A (E7J5D) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 25 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
MS4A4A (E7J5D) Rabbit mAb recognizes endogenous levels of total human MS4A4A protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala228 of human MS4A4A protein.
Background
Membrane-spanning 4-domains subfamily A member 4A (MS4A4A) is a member of a class of MS4A genes encoding four-transmembrane-spanning proteins (1,2). MS4A4A expression is enriched in human and mouse macrophages (3,4). Its expression in macrophages may be induced during macrophage development and activation (4). MS4A4A, via its interaction with the β-glucan receptor, Dectin-1, may contribute to macrophage-mediated production of proinflammatory cytokines and reactive oxygen species by activation of the Syk signaling pathway (4). Interestingly, MS4A4A, as well as other members of the MS4A gene cluster, is genetically linked to late-onset Alzheimer’s disease, suggesting that MS4A4A-dependent macrophage/microglia might contribute to neurodegenerative disease progression (5,6).
- Ma, J. et al. (2015) Mol Neurobiol 51, 1240-8.
- Villegas-Llerena, C. et al. (2016) Curr Opin Neurobiol 36, 74-81.
- Sanyal, R. et al. (2017) Immunol Cell Biol 95, 611-9.
- Mattiola, I. et al. (2019) Nat Immunol 20, 1012-22.
- Naj, A.C. et al. (2011) Nat Genet 43, 436-41.
- Deming, Y. et al. (2019) Sci Transl Med 11, eaau2291. doi: 10.1126/scitranslmed.aau2291.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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