MRP2/ABCC2 (R260) Antibody #4446
Filter:
- WB
- IP
- IF
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | > 200 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:800 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
MRP2/ABCC2 (R260) Antibody detects endogenous levels of total MRP2 protein.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg260 of human MRP2 protein. Antibodies were purified by protein A and peptide affinity chromatography.
Background
Multi-drug resistance protein 2 (MRP2), also known as cMRP, cMOAT, and ABCC2, is an ATP binding cassette (ABC) transporter and part of the multi-drug resistance (MRP) family (1,2). The MRP proteins are membrane proteins that function as organic anion pumps involved in the cellular removal of cancer drugs (2). MRP2 is associated with resistance to a number of cancer drugs, such as cisplatin, etoposide, doxorubicin, and methotrexate (3-5). MRP2 is predominately expressed on the apical membranes in the liver (6-9) and kidney proximal tubules (10). It is responsible for the ATP-dependent secretion of bilirubin glucuronides and other organic anions from hepatocytes into the bile, a process important for the excretion of endogenous and xenobiotic substances. Loss of MRP2 activity is the cause of Dubin-Johnson syndrome, an autosomal recessive disorder characterized by defects in the secretion of anionic conjugates and the presence of melanin like pigments in hepatocytes (11-13).
- Keppler, D. and Konig, J. (1997) FASEB J 11, 509-16.
- Borst, P. et al. (2000) J Natl Cancer Inst 92, 1295-302.
- Taniguchi, K. et al. (1996) Cancer Res 56, 4124-9.
- Hooijberg, J.H. et al. (1999) Cancer Res 59, 2532-5.
- Cui, Y. et al. (1999) Mol Pharmacol 55, 929-37.
- Büchler, M. et al. (1996) J Biol Chem 271, 15091-8.
- Paulusma, C.C. et al. (1996) Science 271, 1126-8.
- Mayer, R. et al. (1995) J Cell Biol 131, 137-50.
- Ito, K. et al. (1998) J Biol Chem 273, 1684-8.
- Schaub, T.P. et al. (1997) J Am Soc Nephrol 8, 1213-21.
- Dubin, I.N. and Johnson, F.B. (1954) Medicine (Baltimore) 33, 155-97.
- Kartenbeck, J. et al. (1996) Hepatology 23, 1061-6.
- Paulusma, C.C. et al. (1997) Hepatology 25, 1539-42.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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