R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
MPP8 (F4Z4O) Rabbit mAb #28139
Filter:
- WB
- IP
- ChIP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 120 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Chromatin IP | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
MPP8 (F4Z4O) Rabbit mAb recognizes endogenous levels of total MPP8 protein. This antibody detects a 13 kDa band of unknown origin.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro246 of human MPP8 protein.
Background
MPP8, also known as MPHOSPH8, is a methylated H3K9 reader protein that interacts with histone methyltransferases ESET and GLP (1). The N-terminal chromodomain specifically recognizes di- and tri-methyl H3K9 over methylated H3K27 and can be phosphorylated, contributing to its binding activity (2,3). MPP8 forms a dimer that serves as a bridge between G9a, GLP, and DNMT3A to set up cytosine methylation and H3K9 tri-methylation at target loci (4). MPP8 is a member of the human silencing hub (HUSH) complex along with TASOR, PPHLN1, and MORC2, which localizes to H3K9me3 sites and cooperates with SETDB1 to maintain H3K9me3 levels and transcriptional repression (5). The HUSH complex is capable of silencing long interspersed elements (LINEs) located in euchromatic regions (6). The HUSH complex can also play a role in the viral life cycle by silencing transcript production from viral genomes (7).
- Kokura, K. et al. (2010) EMBO J 29, 3673-87.
- Chang, Y. et al. (2011) J Mol Biol 408, 807-14.
- Li, J. et al. (2011) PLoS One 6, e25104.
- Chang, Y. et al. (2011) Nat Commun 2, 533.
- Tchasovnikarova, I.A. et al. (2015) Science 348, 1481-1485.
- Liu, N. et al. (2018) Nature 553, 228-232.
- Das, A. et al. (2022) J Virol 96, e0203921.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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