R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
MPC1 (D2L9I) Rabbit mAb #14462
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 12 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
MPC1 (D2L9I) Rabbit mAb recognizes endogenous levels of total MPC1 protein. This antibody does not cross-react with MPC2 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MPC1 protein.
Background
The transport of the glycolytic end product pyruvate into mitochondria and the decarboxylation of pyruvate in the citric acid cycle generate energy through oxidative phosphorylation under aerobic conditions (1,2). Two inner mitochondrial membrane proteins, mitochondrial pyruvate carrier 1 (MPC1) and mitochondrial pyruvate carrier 2 (MPC2), form a 150 kDa complex and are essential proteins in the facilitated transport of pyruvate into mitochondria (1,2). Mutations in the corresponding MPC1 gene are associated with deficient pyruvate transport and may result in lactic acidosis, developmental delay, and premature death (2,3). Altered MPC1/MPC2 expression or activity may result in significant metabolic disorders and contribute to the increase in aerobic glycolysis in cancer cells (a.k.a., the Warburg effect) (4).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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