MOB1 Antibody #3863
Filter:
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 24 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
MOB1 Antibody detects endogenous levels of total MOB1 protein (MOB1α and MOB1β).
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro15 of human MOB1. Antibodies are purified by protein A and peptide affinity chromatography.
Background
MOB1 was first identified in yeast as a protein that binds to Mps with essential roles in the completion of mitosis and the maintenance of ploidy (1). Its Drosophila and mammalian homologs, Mats and MOB1, respectively, are involved in the Hippo signaling tumor suppressor pathway, which plays a critical role in organ size regulation and which has been implicated in cancer development (2-5). There are two MOB1 proteins in humans, MOB1A and MOB1B, that are encoded by two different genes but which have greater than 95% amino acid sequence identity (6). Both forms bind to members of the nuclear Dbf2-related (NDR) kinases, such as LATS1/2 and NDR1/2, thereby stimulating kinase activity (7-9). This binding is promoted by the phosphorylation of MOB1 at several threonine residues (e.g., Thr12, Thr35) by MST1 and/or MST2 (5,10).
- Luca, F.C. and Winey, M. (1998) Mol Biol Cell 9, 29-46.
- Edgar, B.A. (2006) Cell 124, 267-73.
- Saucedo, L.J. and Edgar, B.A. (2007) Nat Rev Mol Cell Biol 8, 613-21.
- Harvey, K. and Tapon, N. (2007) Nat Rev Cancer 7, 182-91.
- Zeng, Q. and Hong, W. (2008) Cancer Cell 13, 188-92.
- Praskova, M. et al. (2008) Curr Biol 18, 311-21.
- Devroe, E. et al. (2004) J Biol Chem 279, 24444-51.
- Hergovich, A. et al. (2005) Mol Cell Biol 25, 8259-72.
- Hergovich, A. et al. (2006) Biochem Biophys Res Commun 345, 50-8.
- Hirabayashi, S. et al. (2008) Oncogene 27, 4281-92.
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
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For Research Use Only. Not For Use In Diagnostic Procedures.
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