R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
mNeonGreen Tag (E8E3V) Rabbit mAb #55074
Filter:
- WB
Supporting Data
| REACTIVITY | All |
| SENSITIVITY | Transfected Only |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
mNeonGreen Tag (E8E3V) Rabbit mAb detects recombinant proteins containing mNeonGreen protein. The antibody recognizes mNeonGreen protein fused to either the amino or carboxy terminus of targeted proteins in transfected cells. mNeonGreen Tag (E8E3V) Rabbit mAb detects exogenously expressed mNeonGreen protein in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated. This antibody does not cross-react with green fluorescent protein (GFP).
Species Reactivity:
All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu18 of mNeonGreen protein.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.
mNeonGreen is a 220 amino acid, bright green fluorescent protein derived from Branchiostoma lanceolatum. mNeonGreen is a very useful tool as a fusion protein that reports gene expression, traces cell lineage, and defines subcellular protein localization (1).
mNeonGreen is a 220 amino acid, bright green fluorescent protein derived from Branchiostoma lanceolatum. mNeonGreen is a very useful tool as a fusion protein that reports gene expression, traces cell lineage, and defines subcellular protein localization (1).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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