R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
MMP-8 (E1C4L) Rabbit mAb #78399
Filter:
- WB
- IP
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous (IHC-P), Transfected (W) |
| MW (kDa) | 70 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:200 - 1:800 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
MMP-8 (E1C4L) Rabbit mAb recognizes endogenous levels of total MMP-8 protein by IHC-P and transfected levels of MMP-8 protein by Western blot. Non-specific staining was observed in human salivary gland by immunohistochemistry.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human MMP-8 protein.
Background
The matrix metalloproteinases (MMPs) are a family of proteases that target many extracellular proteins including other proteases, growth factors, cell surface receptors, and adhesion molecules (1). Among the family members, MMP-2, MMP-3, MMP-7, and MMP-9 have been characterized as important factors for normal tissue remodeling during embryonic development, wound healing, tumor invasion, angiogenesis, carcinogenesis, and apoptosis (2-4). Research studies have shown that MMP activity correlates with cancer development (2). One mechanism of MMP regulation is transcriptional (5). Once synthesized, MMP exists as a latent proenzyme. Maximum MMP activity requires proteolytic cleavage to generate active MMPs by releasing the inhibitory propeptide domain from the full-length protein (5). MMP-8 is also known as Neutrophil Collagenase as it is stored in special granules in neutrophils. Like other MMPs, it also cleaves fibrillar collagens. It also is implicated in various cancers and is thought to be pro-tumorigenic (6).
- McCawley, L.J. and Matrisian, L.M. (2001) Curr Opin Cell Biol 13, 534-40.
- Coussens, L.M. et al. (2002) Science 295, 2387-92.
- Sternlicht, M.D. et al. (1999) Cell 98, 137-46.
- Vu, T.H. et al. (1998) Cell 93, 411-22.
- Nagase, H. et al. (1990) Biochemistry 29, 5783-9.
- Juurikka, K. et al. (2019) Int J Mol Sci 20, .
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