MLH1 (4C9C7) Mouse mAb #3515
Filter:
- WB
- IP
- IF
- F
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 85 |
| Source/Isotype | Mouse IgG1 |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:300 |
| Flow Cytometry (Fixed/Permeabilized) | 1:400 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
MLH1 (4C9C7) Mouse mAb detects endogenous levels of total MLH1 protein.
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with truncated recombinant MBP-MLH1.
Background
Mismatch repair (MMR), a conserved process that involves correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity. MLH1 is the human homologue of the E. coli MMR gene mutL. MMR requires recognition of a base mismatch or insertion/deletion loop by a MutS homolog followed by recruitment of a MutL heterodimeric complex consisting of MLH1 and PMS1 (MutL-γ), PMS2 (MutL-α), or MLH3 (MutL-γ). Other factors required for MMR in eukaryotes are EXO1, PCNA, RFC, RPA, DNA polymerases, and DNA ligases (reviewed in 1). Inactivation of the MLH1 gene causes genome instability and predisposition to cancer (2-5). The MLH1 gene is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC) (6). MLH1 also plays a role in meiotic recombination (7).
- Modrich, P. (2006) J Biol Chem 281, 30305-9.
- Seng, T.J. et al. (2008) Br J Cancer 99, 375-82.
- Harley, I. et al. (2008) Gynecol Oncol 109, 384-7.
- Mao, G. et al. (2008) J Biol Chem 283, 3211-6.
- Hubner, R.A. and Houlston, R.S. (2007) J Natl Cancer Inst 99, 1490; author reply 1490-1.
- Vasen, H.F. (2005) Fam Cancer 4, 219-25.
- Argueso, J.L. et al. (2003) Mol Cell Biol 23, 873-86.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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