R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
MKL1/MRTF-A (E2V2I) Rabbit mAb #97109
Filter:
- WB
- IP
- ChIP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 145 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
For optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 × 10^6 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Chromatin IP | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
MKL1/MRTF-A (E2V2I) Rabbit mAb recognizes endogenous levels of total MKL1/MRTF-A protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly812 of human MKL1/MRTF-A protein.
Background
The megakaryoblastic leukemia proteins 1 and 2 (MKL1, MKL2) are myocardin-related transcription factors (MRTF-A, MRTF-B) that serve as actin-regulated transcription coactivators for the serum response factor (SRF). Interaction between G-actin and MKL proteins retains the coactivator within the cytoplasm of resting cells. Activated Rho-A promotes F-actin assembly and a reduction of the G-actin pool in serum-stimulated cells. This results in the accumulation of MKL proteins in the nucleus, where the coactivator associates with the SRF to activate target gene transcription and mediate multiple cellular processes (1-4). A number of other signaling pathways, including the TGFβ, BMP, and PDGF pathways, also make use of MKL-mediated activation of target gene transcription (5-9). Chromosomal translocations involving the genes encoding MKL1 and MKL2 have been identified in several cases of acute megakaryoblastic leukemia and chondroid lipoma (10-12).
- Olson, E.N. and Nordheim, A. (2010) Nat Rev Mol Cell Biol 11, 353-65.
- Knöll, B. (2010) Biol Chem 391, 591-7.
- Cen, B. et al. (2004) J Cell Biochem 93, 74-82.
- Pipes, G.C. et al. (2006) Genes Dev 20, 1545-56.
- O'Connor, J.W. and Gomez, E.W. (2013) PLoS One 8, e83188.
- Scharenberg, M.A. et al. (2014) J Cell Sci 127, 1079-91.
- Wang, D. et al. (2012) J Biol Chem 287, 28067-77.
- Lundquist, M.R. et al. (2014) Cell 156, 563-76.
- Vasudevan, H.N. and Soriano, P. (2014) Dev Cell 31, 332-44.
- Huang, D. et al. (2010) Genes Chromosomes Cancer 49, 810-8.
- Flucke, U. et al. (2013) Histopathology 62, 925-30.
- Ma, Z. et al. (2001) Nat Genet 28, 220-1.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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