R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Microcephalin-1/BRIT1 (D38G5) Rabbit mAb #4120
Filter:
- WB
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 100 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Microcephalin-1/BRIT1 (D38G5) Rabbit mAb detects endogenous levels of total Microcephalin-1/BRIT1 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to central residues of human Microcephalin-1/BRIT1.
Background
Microcephalin-1 (MCPH1)/BRIT1 is an early DNA damage response protein named for its mutated state in the human disease primary microcephaly. BRIT1 forms damage-induced nuclear foci, is involved in DNA damage and cell cycle checkpoints as well as regulation of mitosis. BRIT1 function is necessary for DNA damage responses, and the absence of BRIT1 function leads to genome instability. A potential tumor suppressor, BRIT1 expression is reduced in human carcinomas (1-2, reviewed in 3).
BRIT1 colocalizes with other DNA repair proteins (53BP1, MDC1, NBS1, ATM, RPA, and ATR) and is required for their activation (2). BRIT1 likely regulates DNA repair through chromatin remodeling in response to DNA damage, allowing access of repair proteins to DNA (4).
BRIT1 colocalizes with other DNA repair proteins (53BP1, MDC1, NBS1, ATM, RPA, and ATR) and is required for their activation (2). BRIT1 likely regulates DNA repair through chromatin remodeling in response to DNA damage, allowing access of repair proteins to DNA (4).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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