R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
MICAL1 (D8K7R) Rabbit mAb #41112
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 130 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
MICAL1 (D8K7R) Rabbit mAb recognizes endogenous levels of total MICAL1 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val1038 of human MICAL1 protein.
Background
Molecule interacting with CasL protein 1 (MICAL1) is a Protein-methionine sulfoxide oxidase. MICAL1 can bind directly to F-actin and oxidize specific methionine residues to promote actin filament disassembly (1-3). MICAL1 is an important component of semaphorin signaling cascades that has effects on cell movement, angiogenesis, immunology, diabetes, and cancer (2,4-6). MICAL1 binds to NDR1/2 and antagonizes MST1-induced NDR activation and apoptosis (7).
- Hung, R.J. et al. (2013) Nat Cell Biol 15, 1445-54.
- Lee, B.C. et al. (2013) Mol Cell 51, 397-404.
- Giridharan, S.S. and Caplan, S. (2014) Antioxid Redox Signal 20, 2059-73.
- Terman, J.R. et al. (2002) Cell 109, 887-900.
- Aggarwal, P.K. et al. (2015) Diabetes 64, 1743-59.
- Loria, R. et al. (2015) Oncotarget 6, 2779-93.
- Zhou, Y. et al. (2011) Mol Cell Biol 31, 3603-15.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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