R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
mGluR5 (D6E7B) Rabbit mAb #55920
Filter:
- WB
- IP
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 150, 300 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Frozen) | 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
mGluR5 (D6E7B) Rabbit mAb recognizes endogenous levels of total mGluR5 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human mGluR5 protein.
Background
mGluR5, a metabotropic glutamate receptor, is a class C G protein-coupled receptor that signals through the Gaq/11-PLC-inositol 1,4,5 triphosphate pathway (1). mGluR5 is comprised of a large N-terminal extracellular domain, seven transmembrane domains, and a C-terminal intracellular domain. Glutamate binding to mGluR5 leads to an increase in intracellular calcium levels and stimulation of PKC activity (2). In neurons, mGluR5 is found in the post-synapse, in a complex with NMDA receptors, PSD-95, SHANK, and Homer (3). mGluR5 is also expressed in microglia and astrocytes (4). Neuronal mGluR5 has been shown to interact with amyloid beta oligomers, and mGluR5 antagonists exhibit neuroprotective effects (5) placing mGluR5 as a potential therapeutic target for Alzheimer’s disease. In glial cells, mGluR5 appears to play an anti-inflammatory role by negatively regulating the release of inflammatory factors (6).
- Abdul-Ghani, M.A. et al. (1996) J Neurophysiol 76, 2691-700.
- Pin, J.P. and Duvoisin, R. (1995) Neuropharmacology 34, 1-26.
- Tu, J.C. et al. (1999) Neuron 23, 583-92.
- Verkhratsky, A. et al. (1998) Physiol Rev 78, 99-141.
- Bruno, V. et al. (2000) Neuropharmacology 39, 2223-30.
- Byrnes, K.R. et al. (2009) Glia 57, 550-60.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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