R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
mGluR1 (D5H10) Rabbit mAb #12551
Filter:
- WB
- IP
- IHC
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 145, >300 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
| Immunofluorescence (Frozen) | 1:400 - 1:1600 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
mGluR1 (D5H10) Rabbit mAb recognizes endogenous levels of total mGluR1 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu1105 of human mGluR1 protein.
Background
Metabotropic glutamate receptor 1 (mGluR1) is a G protein-coupled receptor (GPCR) for the neurotransmitter glutamate in the mammalian brain. Unlike ionotropic receptors, metabotropic receptors do not form an ion channel pore themselves but are indirectly linked to ion channels (1). Both mGluR1 and mGluR5 are coupled to phospholipase C and activate inositol phospholipid metabolism via G protein-mediated mechanisms. Upon phosphatidylinositol activation, the second messenger calcium is released and generates a calcium-activated chloride current. Metabotropic glutamate receptors other than mGluR1 and mGluR5 inhibit adenylate cyclase (1-3). mGluR1 does not share sequence homology with conventional GPCRs (1). mGluR1 forms a homodimer and is linked to synaptic plasticity, as well as long-term potentiation and long-term depression. Furthermore, mGluR1 is a potential therapeutic target for various psychiatric and neurological diseases, including schizophrenia, epilepsy, and Parkinson and Alzheimer diseases (4-6).
- Pin, J.P. et al. (1994) EMBO J 13, 342-8.
- Sugiyama, H. et al. (1987) Nature 325, 531-3.
- Hermans, E. and Challiss, R.A. (2001) Biochem J 359, 465-84.
- Niswender, C.M. et al. (2005) Curr Top Med Chem 5, 847-57.
- Pellicciari, R. and Costantino, G. (1999) Curr Opin Chem Biol 3, 433-40.
- Olive, M.F. (2009) Curr Drug Abuse Rev 2, 83-989.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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