R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
METTL5 (F4E9V) Rabbit mAb #78409
Filter:
- WB
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 26 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
METTL5 (F4E9V) Rabbit mAb recognizes endogenous levels of total METTL5 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu13 of human METTL5 protein.
Background
Methyltransferase-like protein 5 (METTL5) forms a stable complex with TRMT112 in order to catalyze N6-methyladenosine on the 18S rRNA at position 1832 (1). METTL5 can affect the global translation rate, pluripotency, differentiation, and neural development (2-4). High expression of METTL5 has been described in lung adenocarcinoma where it contributes to epithelial-mesenchymal transition (5,6). In pancreatic cancer, METTL5 promotes Myc translation to drive progression (6).
- van Tran, N. et al. (2019) Nucleic Acids Res 47, 7719-7733.
- Ignatova, V.V. et al. (2020) Genes Dev 34, 715-729.
- Wang, L. et al. (2022) Genes Dis 9, 268-274.
- Xing, M. et al. (2020) EMBO Rep 21, e49863.
- Sun, S. et al. (2020) Front Genet 11, 617174.
- Huang, H. et al. (2022) Int J Oncol 60, 9. doi: 10.3892/ijo.2021.5299.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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