R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Metavinculin (F3R2D) Rabbit mAb #64558
Filter:
- WB
- IP
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 145 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:200 - 1:800 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Metavinculin (F3R2D) Rabbit mAb recognizes endogenous levels of total metavinculin protein. Based on the sequence of the peptide antigen, this antibody is not expected to detect vinculin.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro943 of human metavinculin protein.
Background
Vinculin is a cytoskeletal protein localized to cell-matrix and cell-cell adhesion complexes with roles in cell adhesion, migration, and signal transduction (1). Metavinculin is a vinculin isoform resulting from alternative splicing containing a 68-residue insert (2). Metavinculin is expressed in cardiac and smooth muscle (3), and mutations in the metavinculin insert are associated with various cardiomyopathies (4). Metavinculin protein functions in the cytoskeleton with roles in actin bundling, focal adhesion formation, and cell adhesion and migration (5-7).
- Bays, J.L. and DeMali, K.A. (2017) Cell Mol Life Sci 74, 2999-3009.
- Byrne, B.J. et al. (1992) J Biol Chem 267, 12845-50.
- Feramisco, J.R. et al. (1982) J Biol Chem 257, 11024-31.
- Zemljic-Harpf, A. et al. (2009) J Investig Med 57, 849-55.
- Kim, L.Y. et al. (2016) J Mol Biol 428, 10-25.
- Lee, H.T. et al. (2019) PLoS One 14, e0221962.
- Kanoldt, V. et al. (2020) Nat Commun 11, 6403.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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