This product is the carrier-free version of product #99966. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

Formulation

Supplied in 1X PBS (10 mM Na₂HPO₄, 3 mM KCl, 2 mM KH₂PO₄, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

For standard formulation of this product see product #99966.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

Mesothelin (D9R5G) XP^® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total mesothelin protein. The antibody detects both uncleaved (full-length) and cleaved forms of mesothelin, but does not detect the cleaved fragment corresponding to megakaryocyte potentiating factor (MPF).

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human mesothelin protein.

Background

The MSLN gene encodes a 69 kDa precursor protein that is proteolytically cleaved to yield megakaryocyte potentiating factor (MPF) and a GPI-anchored membrane protein termed mesothelin (1). Expression of (cleaved) mesothelin is largely confined to mesothelial cells of normal pleura, pericardium, and peritoneum, but has been reported to be overexpressed in some cancers, including mesothelioma, and some pancreatic and ovarian adenocarcinomas (1,2). Although suggested to be involved in cell adhesion, the physiological functions of mesothelin have not been determined. It is known, however, that mesothelin can be shed from the cell surface following cleavage by TNF-α converting enzyme. Research studies show that serum levels of mesothelin are markedly increased in patients with mesothelioma and ovarian cancer (1), suggesting that serum mesothelin levels may have utility as a cancer biomarker (1-3).

Database Links

UniProt ID: Q13421

Entrez-Gene Id: 10232

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受，否书均被拒书，并且无效。

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Mesothelin (D9R5G) XP® Rabbit mAb (BSA and Azide Free) #50126

Supporting Data

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Product Information

Product Usage Information