Mena Antibody #2075
Filter:
- WB
Supporting Data
REACTIVITY | H M R |
SENSITIVITY | Endogenous |
MW (kDa) | 80, 88, 140 |
SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Mena Antibody detects endogenous levels of total Mena protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide of human Mena. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Mena, EVL, and VASP are all members of the Ena/VASP family, which is involved in controlling cell shape and cell movement by shielding actin filaments from capping proteins (1). Ena/VASP proteins have three distinct domains: an amino-terminal EVH1 domain controlling protein localization; a central proline-rich domain mediating interactions with SH3 and WW domain-containing proteins, including profilin; and a carboxy-terminal domain that promotes tetramerization and actin-binding (2). Mena (also known as ENAH or Protein enabled homolog), interacts with actin filaments at the growing ends and is thus localized to lamellipodia and the tips of neuronal growth cone filopodia. Axons projecting from interhemispheric cortico-cortical neurons were shown to be misrouted in newborn, homozygous Mena knockout mice (3). Mena may be phosphorylated at Ser236 by PKA, a post-translational modification (PTM) that is reported to promote filopodial formation and elongation of the growth cone (4). Three forms of the Mena protein, with apparent molecular weights of 80, 88, and 140 kDa, have been described. The 80 kDa isoform is broadly expressed, whereas the 140 kDa isoform is reportedly enriched in neural cell types; these isoforms are generated by alternative splicing. The 88 kDa isoform is expressed primarily in embryonic cells and is likely the result of PTM of the 80 kDa isoform. Expression of all three forms is completely eliminated after homozygous deletion of ENAH, the gene encoding the Mena protein (1,3).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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